Computational Advances in Structural Biology

Abstract :

Subtomogram averaging from cryo electron tomograms is a powerful method to determine structures of macromolecules in their native state. Outstanding applications to protein lattices, coats and ribosomes provided unique insights into their functions and even revealed interactions with small molecules in situ. For other macromolecules, such as membrane proteins, which are present in tomograms in limited numbers, the throughput of data processing and the processing time are key bottlenecks in obtaining high-resolution reconstructions. This is particularly the case for membrane proteins that are typically present in tomograms in moderate amounts.